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Dr David A Dougan
Department of Biochemistry
La Trobe University
Victoria, 3086
Email: d.dougan@latrobe.edu.au
Tel: +61 3 9479 3276
Fax: +61 3 9479 2467
Homepage: http://www.latrobe.edu.au/biochemistry/labs/dougan/research.html
| Research |
| Protein quality control is essential for the proper maintenance of all living cells. It ensures, not only the correct folding of newly synthesised proteins, the refolding or degradation of misfolded and aggregated proteins, but also controls the degradation of regulatory proteins. Collectively, these functions are performed by molecular chaperones and proteases. In our laboratory we are primarily interested in proteases from the bacterial cytosol and the mitochondrial matrix. Our current research concentrates on defining the molecular basis of substrate selectivity for several different ATP-dependent proteases. A major focus of the work is directed towards the mechanistic analysis of novel AAA+ adaptor proteins such as ClpS, which we recently discovered and how these protein co-factors modulate AAA+ substrate specificity. A detailed analysis of protease substrate specificity is central to our understanding of how chaperones and protease compete for different substrates in vivo. |
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| Collaborations |
Bernd Bukau (ZMBH, Heidelberg, Germany)
Tim Clausen (Research Institute of Molecular Pathology, Vienna, Austria)
Art Horwich (Yale University, Connecticut, U.S.A.)
Bernard Guiard (CNRS Centre de Genetique Moleculaire, Gif-sur-Yvette, France)
Klaus Pfanner (University of Freiburg, Germany)
Neil Ranson (University of Leeds, U.K.)
Kursad Turgay (University of Heidelberg, Germany)
Eilika Weber-Ban (ETH, Zurich, Switzerland)
Kornelius Zeth (MPI for Biochemistry, Martinsried, Germany)
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| Publications |
Dougan D.A., Reid B.G., Horwich A.L. & Bukau B. (2002). ClpS, a substrate modulator of the ClpAP machine. Mol. Cell 9, 973-983.
Zeth K., Ravelli R., Paal K., Cusack S., Bukau B. & Dougan D.A. (2002). Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA. Nature Struct. Biol. 9, 906-911.
Dougan D.A., Mogk A., Zeth K., Turgay K. and Bukau B. (2002). AAA+ proteins and substrate recognition, it all depends on their partner in crime. FEBS Letters 529, 6-10.
Schlothauer T., Mogk A., Dougan D.A., Bukau B. & Turgay K. (2003). MecA and ClpC of Bacillus subtilis form a new bipartite chaperone system. Proc. Natl. Acad. Sci. U.S.A. 100, 2306-2311.
Dougan D.A., Weber-Ban E. & Bukau, B (2003). Targeted delivery of an ssrA-tagged substrate by the adaptor protein SspB to its cognate AAA+ protein ClpX. Mol. Cell 12, 373-380.
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