|
|
|
Dr Jonathan M Harris
School of Life Science
Queensland University of Technology
Brisbane , Queensland 4000
Email: j2.harris@qut.edu.au
Tel: +61-7-3864 5149
Fax:
Homepage:
| Research |
| We use a combination of molecular modelling, peptide synthesis and protein expression to understand structure/function relationships in diverse proteins ranging from transcription factors to signal transduction molecules. Since moving to the Queensland University of technology we have been involved in screening phage display libraries for potential novel inhibitors of serine proteases. Additionally, we have created molecular models of the Kallikrein serine proteases and have produced milligram quantities of purified recombinant protein for phage display screening and antibody production. Currently we are engineering the yeast Pichia pastoris to allow production of large quantities of recombinant serine proteases for substrate identification and crystallography. Yeast expression will allow production of cysteine rich proteins such as the kallikrein proteases. |
|
| Collaborations |
Professor Judith Clements-Queensland University of Technology
Professor Adrian Herington-Queensland University of Technology
Dr Bostjan Kobe-University of Queensland
|
|
| Publications |
Buchanan G, Greenberg NM, Scher HI, Harris JM, Marshall VR, Tilley WD."Collocation of androgen receptor gene mutations in prostate cancer." (2001) Clin Cancer Res. 5:1273-81.
Harris, J.M., Lau, P., Chen, S-L, and Muscat G.E.O. "Characterization of the RORalpha coactivator binding interface: a structural basis for ligand independent transcription" (2002) Molecular Endocrinol 16, 998-1012.
Wansa, K. D., Harris, J. M. & Muscat, G. E. “The activation function-1 domain of Nur77/NR4A1 mediates trans-activation, cell specificity, and coactivator recruitment” (2002) J Biol Chem 277, 33001-11.
Alaina J. James, I. U. A., Jonathan M. Harris, Grant Buchanan, Wayne D. Tilley, Marco Marcelli, Dolores J. Lamb, and Nancy & Weigel, L. “A novel androgen receptor mutant, A748T, exhibits hormone concentration-dependent defects in nuclear accumulation and activity despite normal hormone-binding affinity” (2003) Molecular Endocrinology Published online ahead of print
Veveris-Lowe, T., Harris, J.M., Ya, A.S., Nicol, D.L. and Clements, J.A. Prostate-specific antigen (PSA) over-expression alters cell morphology and the invasive and migratory capacity of PC-3 cells. The Endocrine Society’s 84th Annual Meeting, San Francisco 2002 and the Endocrine Society of Australia 45th Annual Scientific meeting, Adelaide 2002.
|
|
|
|
|
|
|
|
|
